Proteintech, 86199-3-RR, DUSP9 Recombinant monoclonal antibody

Size: 20ul / 100ul The DUSP9 (86199-3-RR) by Proteintech is a Recombinant antibody targeting DUSP9 in WB, ELISA applications with reactivity to human samples 86199-3-RR targets DUSP9 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HuH-7 cells, HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Dual specificity protein phosphatase 9 (DUSP9) is also named itogen-activated protein kinase phosphatase 4 (MAP kinase phosphatase 4; MKP-4). DUSP9 is a typical DUSP characterized by the presence of an MKB/KIM motif and a phosphatase domain, which shares structural homology with other DUSPs (PMID: 25519881). DUSP9 is an important downstream regulator of BMP/Smad signaling and a key factor in maintaining the stemness of mESCs (PMID: 34768967). DUSP9 is one of the key genes involved in gonadotrophin-mediated ovarian follicle development (PMID: 33676987). DUSP9 expression increases during insulin-induced adipogenesis and culminates in mature adipocytes. DUSP9 as a key regulator of insulin signaling and highlighted its potential role in insulin resistance and metabolic diseases by dephosphorylating kinases involved in metabolic processes, glucose uptake and storage. DUSP9 can also impair the action of extracellular mediators and stress inducers (i.e., proinflammatory cytokines), which can induce insulin resistance by abnormally activating MAPK or SAP pathways (PMID: 30063256, PMID: 18296638). In normal mature tissues, DUSP9 is mainly expressed in kidney, adipose tissue and placenta, while it is only minimally present in brain, ovary, testis and urinary bladder (PMID: 34768967). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag25167 Product name: Recombinant human DUSP9 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 343-384 aa of BC060837 Sequence: SLRLEERHSQEQGSGGQASAASNPPSFFTTPTSDGAFELAPT Predict reactive species Full Name: dual specificity phosphatase 9 Calculated Molecular Weight: 42 kDa Observed Molecular Weight: 42 kDa GenBank Accession Number: BC060837 Gene Symbol: DUSP9 Gene ID (NCBI): 1852 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q99956 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.