Proteintech, 86307-1-RR, Phospho-Cofilin (Ser3) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-Cofilin (Ser3) (86307-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-Cofilin (Ser3) in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 86307-1-RR targets Phospho-Cofilin (Ser3) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, NIH/3T3 cells, λ phosphatase treated HeLa cells, λ phosphatase treated NIH/3T3 cells Positive IF/ICC detected in: λ phosphatase treated Hela cells Positive FC (Intra) detected in: λ phosphatase treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of the ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: cofilin 1 (non-muscle) Calculated Molecular Weight: 18 kDa Observed Molecular Weight: 18 kDa GenBank Accession Number: BC012318 Gene Symbol: Cofilin Gene ID (NCBI): 1072 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P23528 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.