Proteintech, 86330-1-RR, Phospho-ATR (Ser428) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-ATR (Ser428) (86330-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-ATR (Ser428) in WB, IF/ICC, ELISA applications with reactivity to human samples 86330-1-RR targets Phospho-ATR (Ser428) in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Ataxia-telangiectasia and Rad3 related (ATR) is a serine/threonine kinase and a member of the phosphatidylinositol 3-kinase-related kinase (PIKK) family, particularly the ataxia telangiectasia mutated (ATM) subfamily. The phosphorylation site of ATR is located at Ser428 and is crucial for proper ATR function. In a series of breast cancer studies, high ATR expression and activation were significantly associated with higher tumor stage, mitotic index, pleomorphism, lymphovascular invasion, and poor survival. (PMID: 33854565) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ataxia telangiectasia and Rad3 related Calculated Molecular Weight: 301 kDa Observed Molecular Weight: 300 kDa GenBank Accession Number: NM_001184 Gene Symbol: ATR Gene ID (NCBI): 545 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q13535 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.