Proteintech, 86361-2-RR, WDR74 Recombinant monoclonal antibody

Size: 20ul / 100ul The WDR74 (86361-2-RR) by Proteintech is a Recombinant antibody targeting WDR74 in WB, IF/ICC, ELISA applications with reactivity to human, mouse samples 86361-2-RR targets WDR74 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HepG2 cells, MDA-MB-231 cells, A-673 cells, A375 cells, IMR-32 cells, RAW264.7 cells Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information WDR74, also known as WD repeat domain 74, is a protein-coding gene that plays important roles in various biological processes. WDR74 is involved in rRNA processing and ribosomal large subunit biogenesis. It is a 60S ribosome assembly factor, which is irreplaceable in ribosome biogenesis and is closely related to protein synthesis. WDR74 can induce nuclear β-catenin accumulation and activate Wnt-responsive genes, thus promoting cell proliferation and metastasis in various cancers. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag14617 Product name: Recombinant human WDR74 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC006351 Sequence: MAAAAARWNHVWVGTETGILKGVNLQRKQAANFTAGGQPRREEAVSALCWGTGGETQMLVGCADRTVKHFSTEDGIFQGQRHCPGGEGMFRGLAQADGTLITCVDSGILRVWHDKDKDTSSDPLLELRVGPGVCRMRQDPAHPHVVATGGKENALKIWDLQGSEEPVFRAKNVRNDWLDLRVPIWDQDIQFLPGSQKLVTCTGYHQVRVYDPASPQRRPVLETTYGEYPLTAMTLTPGGNSVIVGNTHGQLAEIDLRQGRLLGCLKGLAGSVRGLQCHPSKPLLASCGLDRVLRIHRIQN Predict reactive species Full Name: WD repeat domain 74 Calculated Molecular Weight: 385 aa, 42 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC006351 Gene Symbol: WDR74 Gene ID (NCBI): 54663 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q6RFH5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.