Proteintech, 86370-1-RR, Pan-Keratin (TypeⅡ) Recombinant monoclonal antibody

Size: 20ul / 100ul The Pan-Keratin (TypeⅡ) (86370-1-RR) by Proteintech is a Recombinant antibody targeting Pan-Keratin (TypeⅡ) in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 86370-1-RR targets Pan-Keratin (TypeⅡ) in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, A431 cells, HT-29 cells, mouse skin tissue, rat colon tissue, rat skin tissue Positive IHC detected in: human stomach cancer tissue, Human Breast Cancer(Her2+;ER-;PR-)Note: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human colon cancer tissue, human cervical cancer tissue, human lung cancer tissue, mouse colon tissue, mouse skin tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:250-1:1000 Background Information Keratin is a family of fibrous structural proteins that are key components in hair, nails, feathers, hooves, and the outer layer of skin (epidermis). There are two main types of keratin: Type I (acidic) and Type II (basic/neutral), which form heterodimers to create intermediate filaments, providing mechanical strength to cells. Type II keratins (or Type II cytokeratins) are basic or neutral proteins that form part of the intermediate filament cytoskeleton in epithelial cells. Type II keratins include K1-K8, and K71-K86. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: Pan-Keratin (TypeⅡ) Observed Molecular Weight: 52-68 kDa Gene ID (NCBI): Pan-Keratin (TypeⅡ) Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q14533 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.