Proteintech, 86393-3-RR, MBD2 Recombinant monoclonal antibody

Size: 20ul / 100ul The MBD2 (86393-3-RR) by Proteintech is a Recombinant antibody targeting MBD2 in WB, ELISA applications with reactivity to human, mouse samples 86393-3-RR targets MBD2 in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A431 cells, HEK-293 cells, HL-60 cells, U266 cells, Jurkat cells, NIH/3T3 cells, RAW 264.7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information MBD2, also named as DMTase, binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. MBD2 binds hemi-methylated DNA as well. It recruits histone deacetylases and DNA methyltransferases. MBD2 acts as transcriptional repressor and plays a role in gene silencing (PMID:9774669). MBD2 belongs to a highly conserved member of the MBD protein family, is considered a reader of DNA methylation and drives the crosstalk between DNA methylation and histone deacetylation (PMID: 40089655). MBD2 drives breast cancer progression through the modulation of epithelial-to-mesenchymal transition (PMID: 38556549). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: methyl-CpG binding domain protein 2 Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 43-50 kDa GenBank Accession Number: NM_003927 Gene Symbol: MBD2 Gene ID (NCBI): 8932 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9UBB5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.