Proteintech, 86398-1-RR, Phospho-Tyrosine Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-Tyrosine (86398-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-Tyrosine in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to - samples 86398-1-RR targets Phospho-Tyrosine in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with - samples. Tested Applications Positive WB detected in: EGF treated A431 cells Positive IF/ICC detected in: hEGF treated A431 cells Positive FC (Intra) detected in: EGF treated A431 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Phospho-Tyrosine antibody refers to a class of pan-specific antibodies or detection reagents that recognize all phosphorylated tyrosine residues regardless of the surrounding amino-acid sequence. These tools are widely used to monitor global tyrosine phosphorylation events in signal-transduction studies, cancer research and drug-discovery screens.This antibody does not cross-react with peptides containing Phospho-Serine or Phospho-Threonine. Specification Tested Reactivity: - Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: Phospho-Tyrosine Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.