Proteintech, 86429-5-RR, ALDH2 Recombinant monoclonal antibody

Size: 20ul / 100ul The ALDH2 (86429-5-RR) by Proteintech is a Recombinant antibody targeting ALDH2 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 86429-5-RR targets ALDH2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, SK-BR-3 cells, HepG2 cells, mouse liver tissue, rat liver tissue Positive IF/ICC detected in: C2C12 cells, A549 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag7452 Product name: Recombinant human ALDH2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 166-517 aa of BC002967 Sequence: DFFSYTRHEPVGVCGQIIPWNFPLLMQAWKLGPALATGNVVVMKVAEQTPLTALYVANLIKEAGFPPGVVNIVPGFGPTAGAAIASHEDVDKVAFTGSTEIGRVIQVAAGSSNLKRVTLELGGKSPNIIMSDADMDWAVEQAHFALFFNQGQCCCAGSRTFVQEDIYDEFVERSVARAKSRVVGNPFDSKTEQGPQVDETQFKKILGYINTGKQEGAKLLCGGGIAADRGYFIQPTVFGDVQDGMTIAKEEIFGPVMQILKFKTIEEVVGRANNSTYGLAAAVFTKDLDKANYLSQALQAGTVWVNCYDVFGAQSPFGGYKMSGSGRELGEYGLQAYTEVKTVTVKVPQKNS Predict reactive species Full Name: aldehyde dehydrogenase 2 family (mitochondrial) Calculated Molecular Weight: 56 kDa Observed Molecular Weight: 50-55 kDa GenBank Accession Number: BC002967 Gene Symbol: ALDH2 Gene ID (NCBI): 217 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P05091 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.