Proteintech, 86444-1-RR, FSTL1 Recombinant monoclonal antibody

Size: 20ul / 100ul The FSTL1 (86444-1-RR) by Proteintech is a Recombinant antibody targeting FSTL1 in WB, ELISA applications with reactivity to human, mouse, rat samples 86444-1-RR targets FSTL1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: 4T1 cells, C6 cells, mouse embryo tissue, A2780 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Follistatin-like 1 (FSTL1), initially discovered as a TGF-β1-induced gene, encodes a 308 amino acid secreted 45-55 kDa glycoprotein with a follistatin domain and two non-functional calcium-binding motifs. FSTL1 has been reported to be involved in the fate determination and maturation of epithelial cells. Ablation of the FSTL1 gene in the mouse results in several structural developmental defects and neonatal lethality due to respiratory failure, demonstrating its functional importance. FSTL1 has been reported to exhibit both pro- and anti-inflammatory actions, with a specific anti-apoptotic and protective effect in cardiac or renal cell injury. Zwijsen et al. (1994) detected several isoforms of FSTL1 with molecular masses of 40 to 48 kD, which differ from the 50- to 55-kD products detected by Tanaka et al. (1998) (OMIM). (PMID: 26386648; PMID: 23723173; PMID: 20176958). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg2859 Product name: Recombinant Mouse FSTL1 protein (rFc Tag) Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 19-306 aa of NM_008047.5 Sequence: EEEPRSKSKICANVFCGAGRECAVTEKGEPTCLCIEQCKPHKRPVCGSNGKTYLNHCELHRDACLTGSKIQVDYDGHCKEKKSASPSASPVVCYQANRDELRRRLIQWLEAEIIPDGWFSKGSNYSEILDKYFKSFDNGDSHLDSSEFLKFVEQNETAINITTYADQENNKLLRSLCVDALIELSDENADWKLSFQEFLKCLNPSFNPPEKKCALEDETYADGAETEVDCNRCVCSCGHWVCTAMTCDGKNQKGVQTHTEEEKTGYVQELQKHQGTAEKTKKVNTKEI Predict reactive species Full Name: follistatin-like 1 Calculated Molecular Weight: 35 kDa Observed Molecular Weight: 35-45 kDa GenBank Accession Number: NM_008047.5 Gene Symbol: Fstl1 Gene ID (NCBI): 14314 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q62356 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.