Proteintech, 86507-3-RR, AGRN Recombinant monoclonal antibody

Size: 20ul / 100ul The AGRN (86507-3-RR) by Proteintech is a Recombinant antibody targeting AGRN in WB, ELISA applications with reactivity to human samples 86507-3-RR targets AGRN in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HaCaT cells, ACHN cells, A431 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information Agrin, a large heparan sulfate proteoglycan, is expressed in neuronal, as well as nonneuronal tissues (PMID:12671652). Nerve-derived agrin has been demonstrated to play an essential role in development and maintenance of the neuromuscular junction (PMID:16386415). The agrin gene encodes a protein of more than 2000 amino acids with predicted molecular weight of 225 kDa but can display as 600 kDa protein due to extensive N-terminal glycosylation (PMID:12671652). Neurotrypsin cleaves the proteoglycan agrin at two homologous, highly conserved sites, liberating a middle 90-kDa and a C-terminal 22-kDa fragment from the membrane or ECM-bound N-terminal moiety of agrin (PMID:18230682). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg5123 Product name: recombinant human Agrin protein Source: mammalian cells -derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 1260-2045 aa of NM_198576.4 Sequence: ATSGAIAAGATARATTASRLPSSAVTPRAPHPSHTSQPVAKTTAAPTTRRPPTTAPSRVPGRRPPAPQQPPKPCDSQPCFHGGTCQDWALGGGFTCSCPAGRGGAVCEKVLGAPVPAFEGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPCQPNPCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPVPHTVLNLKEPLYVGGAPDFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLNGASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPCPTP Predict reactive species Full Name: agrin Calculated Molecular Weight: 215 kDa Observed Molecular Weight: 245 kDa GenBank Accession Number: NM_198576.4 Gene Symbol: AGRN Gene ID (NCBI): 375790 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O00468-6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.