Proteintech, 86547-4-RR, IL-10 Recombinant monoclonal antibody

Size: 20ul / 100ul The IL-10 (86547-4-RR) by Proteintech is a Recombinant antibody targeting IL-10 in WB, ELISA applications with reactivity to human samples 86547-4-RR targets IL-10 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Transfected with Human IL-10 CHO cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg3296 Product name: Recombinant Human IL-10 protein (rFc Tag) Source: mammalian cells -derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 19-178 aa of NM_000572.3 Sequence: SPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN Predict reactive species Full Name: interleukin 10 Calculated Molecular Weight: 21 kDa Observed Molecular Weight: 22 kDa GenBank Accession Number: NM_000572.3 Gene Symbol: IL-10 Gene ID (NCBI): 3586 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P22301 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.