Proteintech, 86697-1-RR, Claudin 3 Recombinant monoclonal antibody

Size: 20ul / 100ul The Claudin 3 (86697-1-RR) by Proteintech is a Recombinant antibody targeting Claudin 3 in WB, IP, ELISA applications with reactivity to human, mouse, rat samples 86697-1-RR targets Claudin 3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HT-29 cells, mouse colon tissue, Calu-3 cells, MCF-7 cells, rat colon tissue Positive IP detected in: HT-29 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information Claudin-3 (CLDN3) is one of 27 known members of the claudin family. Together with occludin and other junctional adhesion molecules, the claudins form the tight-junctions (TJs) that regulate intercellular permeability. Altered CLDN3 expression has been linked to tumor progression in multiple tumor types. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: claudin 3 Calculated Molecular Weight: 220 aa, 23 kDa Observed Molecular Weight: 20 kDa GenBank Accession Number: BC016056 Gene Symbol: Claudin 3 Gene ID (NCBI): 1365 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O15551 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.