Proteintech, 86729-1-RR, Angiotensinogen/AGT Recombinant monoclonal antibody

Size: 20ul / 100ul The Angiotensinogen/AGT (86729-1-RR) by Proteintech is a Recombinant antibody targeting Angiotensinogen/AGT in WB, ELISA applications with reactivity to human samples 86729-1-RR targets Angiotensinogen/AGT in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human plasma, HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Background Information Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene also have been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg3099 Product name: Recombinant Human Angiotensinogen/AGT protein (rFc Tag) Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 25-476 aa of NM_000029.4 Sequence: DRVYIHPFHLVIHNESTCEQLAKANAGKPKDPTFIPAPIQAKTSPVDEKALQDQLVLVAAKLDTEDKLRAAMVGMLANFLGFRIYGMHSELWGVVHGATVLSPTAVFGTLASLYLGALDHTADRLQAILGVPWKDKNCTSRLDAHKVLSALQAVQGLLVAQGRADSQAQLLLSTVVGVFTAPGLHLKQPFVQGLALYTPVVLPRSLDFTELDVAAEKIDRFMQAVTGWKTGCSLMGASVDSTLAFNTYVHFQGKMKGFSLLAEPQEFWVDNSTSVSVPMLSGMGTFQHWSDIQDNFSVTQVPFTESACLLLIQPHYASDLDKVEGLTFQQNSLNWMKKLSPRTIHLTMPQLVLQGSYDLQDLLAQAELPAILHTELNLQKLSNDRIRVGEVLNSIFFELEADEREPTESTQQLNKPEVLEVTLNRPFLFAVYDQSATALHFLGRVANPLSTA Predict reactive species Full Name: angiotensinogen (serpin peptidase inhibitor, clade A, member 8) Calculated Molecular Weight: 52 kDa Observed Molecular Weight: 53 kDa GenBank Accession Number: NM_000029.4 Gene Symbol: AGT Gene ID (NCBI): 183 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P01019 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.