Proteintech, 86731-1-RR, Acetyl-Histone H2B (Lys12) Recombinant monoclonal antibody

Size: 20ul / 100ul The Acetyl-Histone H2B (Lys12) (86731-1-RR) by Proteintech is a Recombinant antibody targeting Acetyl-Histone H2B (Lys12) in WB, IHC, Dot blot, ELISA, ChIP-qPCR applications with reactivity to human, mouse samples 86731-1-RR targets Acetyl-Histone H2B (Lys12) in WB, IHC, Dot blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Trichostatin A treated NIH/3T3 cells, Trichostatin A treated HeLa cells Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive Dot blot detected in: / / Positive ChIP-qPCR detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 DOT BLOT: DOT BLOT : 1:10-1:100 CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: histone cluster 2, H2be Calculated Molecular Weight: 14 kDa Observed Molecular Weight: 15 kDa GenBank Accession Number: BC005827 Gene Symbol: Histone H2B Gene ID (NCBI): 8349 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q16778 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.