Proteintech, 86763-3-RR, DNAJB8 Recombinant monoclonal antibody

Size: 20ul / 100ul The DNAJB8 (86763-3-RR) by Proteintech is a Recombinant antibody targeting DNAJB8 in WB, ELISA applications with reactivity to human, mouse, rat samples 86763-3-RR targets DNAJB8 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: SW480 cells, mouse liver tissue, HeLa cells, HEK-293 cells, mouse brain tissue, rat brain tissue, fetal human brain tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information DNAJB8, a member of the DNAJ/HSP40 family of proteins, plays a crucial role in regulating chaperone HSP70 activity. It is known for its ability to suppress the aggregation of polyglutamine proteins through its C-terminal tail. Furthermore, DNAJB8 has been shown to inhibit TP53 through ubiquitin degradation in human colon cancer by directly interacting and upregulating MDR1, leading to increased oxaliplatin resistance. High levels of DNAJB8 in both colon tissues and serum are correlated with worse overall survival of colon cancer patients, suggesting that DNAJB8 level in serum sEV may serve as a potential biomarker for colon cancer and a promising therapeutic target for oxaliplatin-resistant cancer. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag10899 Product name: Recombinant human DNAJB8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-232 aa of BC029521 Sequence: MANYYEVLGVQASASPEDIKKAYRKLALRWHPDKNPDNKEEAEKKFKLVSEAYEVLSDSKKRSLYDRAGCDSWRAGGGASTPYHSPFDTGYTFRNPEDIFREFFGGLDPFSFEFWDSPFNSDRGGRGHGLRGAFSAGFGEFPAFMEAFSSFNMLGCSGGSHTTFSSTSFGGSSSGSSGFKSVMSSTEMINGHKVTTKRIVENGQERVEVEEDGQLKSVTVNGKEQLKWMDSK Predict reactive species Full Name: DnaJ (Hsp40) homolog, subfamily B, member 8 Calculated Molecular Weight: 232 aa, 26 kDa Observed Molecular Weight: 30 kDa GenBank Accession Number: BC029521 Gene Symbol: DNAJB8 Gene ID (NCBI): 165721 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q8NHS0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.