Proteintech, 86786-1-RR, PARD6B Recombinant monoclonal antibody

Size: 20ul / 100ul The PARD6B (86786-1-RR) by Proteintech is a Recombinant antibody targeting PARD6B in WB, ELISA applications with reactivity to human, mouse, rat samples 86786-1-RR targets PARD6B in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, PC-12 cells, Jurkat cells, HEK-293 cells, T-47D cells, Caki-1 cells, RAW 264.7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information PARD6B (also named PAR6B) is a member of the PAR6 family. PARD6B is known to play a key role in mammary epithelial cell biology. Several studies have been reported which indicate deregulated PARD6B signaling contributes to malignant epithelial cell phenotypes due predominantly to disrupted polymerization and maintenance of tight junctions (PMID: 22957302). The approximately 4.8-kb long PARD6B mRNA was predominantly detected in both adult and fetal kidneys, while much weaker but significant signals were observed in the placenta, lung, and liver (PMID: 11260256). This antibody detects PARD6B with an apparent molecular weight of 50-53 kDa as has been demonstrated by several researches (PMID: 22496418; 25662318). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag5083 Product name: Recombinant human PARD6B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-372 aa of BC060847 Sequence: MNRSHRHGAGSGCLGTMEVKSKFGAEFRRFSLERSKPGKFEEFYGLLQHVHKIPNVDVLVGYADIHGDLLPINNDDNYHKAVSTANPLLRIFIQKKEEADYSAFGTDTLIKKKNVLTNVLRPDNHRKKPHIVISMPQDFRPVSSIIDVDILPETHRRVRLYKYGTEKPLGFYIRDGSSVRVTPHGLEKVPGIFISRLVPGGLAQSTGLLAVNDEVLEVNGIEVSGKSLDQVTDMMIANSRNLIITVRPANQRNNVVRNSRTSGSSGQSTDNSLLGYPQQIEPSFEPEDEDSEEDDIIIEDNGVPQQIPKAVPNTESLESLTQIELSFESGQNGFIPSNEVSLAAIASSSNTEFETHAPDQKLLEEDGTIITL Predict reactive species Full Name: par-6 partitioning defective 6 homolog beta (C. elegans) Calculated Molecular Weight: 41 kDa Observed Molecular Weight: 52 kDa GenBank Accession Number: BC060847 Gene Symbol: PARD6B Gene ID (NCBI): 84612 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9BYG5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.