Proteintech, 86790-1-RR, EPHX2 Recombinant monoclonal antibody

Size: 20ul / 100ul The EPHX2 (86790-1-RR) by Proteintech is a Recombinant antibody targeting EPHX2 in WB, ELISA applications with reactivity to human, mouse samples 86790-1-RR targets EPHX2 in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: U-937 cells, HEK-293 cells, A549 cells, LNCaP cells, Jurkat cells, mouse colon tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Background Information EPHX2(Epoxide hydrolase 2) acts on epoxides (alkene oxides, oxiranes) and arene oxides and plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. A number of single nucleotide polymorphisms (SNPs) in human EPHX2 have been linked to cardiovascular disease risk, including increased risk of coronary heart disease, hyperlipoproteinemia, and type-2 diabetes (PMID: 14732757, 16595607, 14673705, 15845398, 17460077). It was observed in many tissues with the band of 63 kDa in the western blot. It has also been reported that the N-terminal domain might promote dimerization of EPHX2 (PMID:21553642). Negative expression of EPHX2 protein in U-937 cells is consistent with the predicted expression pattern. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag1283 Product name: Recombinant human EPHX2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 212-555 aa of BC013874 Sequence: ELEKVTGIQLLNTPAPLPTSCNPSDMSHGYVTVKPRVRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEEYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANPNMSPLESIKANPVFDYQLYFQEPGVAEAELEQNLSRTFKSLFRASDESVLSMHKVCEAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRKILIPALMVTAEKDFVLVPQMSQHMEDWIPHLKRGHIEDCGHWTQMDKPTEVNQILIKWLDSDARNPPVVSKM Predict reactive species Full Name: epoxide hydrolase 2, cytoplasmic Calculated Molecular Weight: 63 kDa Observed Molecular Weight: 57 kDa GenBank Accession Number: BC013874 Gene Symbol: EPHX2 Gene ID (NCBI): 2053 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P34913 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.