Proteintech, 86791-1-RR, Cardiac Troponin I Recombinant monoclonal antibody

Size: 20ul / 100ul The Cardiac Troponin I (86791-1-RR) by Proteintech is a Recombinant antibody targeting Cardiac Troponin I in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 86791-1-RR targets Cardiac Troponin I in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human heart tissue, mouse heart tissue, rat heart tissue Positive IHC detected in: rat heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse heart tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information Troponin I (TnI) is the inhibitory subunit of troponin, the structural protein involved in the regulation of striated muscle contraction. Cardiac Troponin I (cTnI) is produced by cardiac muscle. cTnI has been considered as one of the most specific and sensitive markers of myocardial damage like acute myocardial infarction (AMI) for decades. Detection of cTnI in serum is widely accepted as a diagnostic tool in acute coronary syndromes. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag16365 Product name: Recombinant human TNNI3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-110 aa of BC096165 Sequence: MADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEE Predict reactive species Full Name: troponin I type 3 (cardiac) Calculated Molecular Weight: 210 aa, 24 kDa Observed Molecular Weight: 24-26 kDa GenBank Accession Number: BC096165 Gene Symbol: Cardiac Troponin I Gene ID (NCBI): 7137 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P19429 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.