Proteintech, 86825-1-RR, P4HA1 Recombinant monoclonal antibody

Size: 20ul / 100ul The P4HA1 (86825-1-RR) by Proteintech is a Recombinant antibody targeting P4HA1 in WB, ELISA applications with reactivity to human samples 86825-1-RR targets P4HA1 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, A431 cells, PC-3 cells, HEK-293 cells, MCF-7 cells, ACHN cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Background Information P4HA1 (Prolyl 4-hydroxylase subunit alpha-1) is also known as P4HA and belongs to the P4HA family, which plays a central role in collagen synthesis. P4HA1 catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. The gene encodes a polypeptide of 517 amino acid residues and a signal peptide of 17 amino acids and the full length protein has two glycosylation sites (uniprot). It can exist as a heteromer, dimer or tetramer (refer to: GENATLAS). P4HA1 has some isoforms with the MW 61-63 kDa and 57-59 kDa. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag3292 Product name: Recombinant human P4HA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 236-534 aa of BC034998 Sequence: PEHQRANGNLKYFEYIMAKEKDVNKSASDDQSDQKTTPKKKGVAVDYLPERQKYEMLCRGEGIKMTPRRQKKLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE Predict reactive species Full Name: prolyl 4-hydroxylase, alpha polypeptide I Calculated Molecular Weight: 534 aa, 61 kDa Observed Molecular Weight: 61 kDa GenBank Accession Number: BC034998 Gene Symbol: P4HA1 Gene ID (NCBI): 5033 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P13674 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.