Proteintech, 86835-1-RR, PGRMC2 Recombinant monoclonal antibody

Size: 20ul / 100ul The PGRMC2 (86835-1-RR) by Proteintech is a Recombinant antibody targeting PGRMC2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 86835-1-RR targets PGRMC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, ARPE-19 cells, HepG2 cells, MDA-MB-231 cells, NIH/3T3 cells, 3T3-L1 cells Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:2500-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information Membrane-associated progesterone receptor component 2 (PGRMC2), also known as DG6 or PMBP, belongs to the cytochrome b5 family. This protein might play a role in cancer. The gene of PGRMC2 maps to chromosome 4q26, and encodes a 223-amino acid single-pass membrane protein with a cytochrome b5 heme-binding domain in its cytoplasmic domain. PGRMC2 has a calculated molecular weight of 24 kDa. The band of about 28 kDa detected by this antibody is unknown but may represent phosphorylated form of PGRMC2 (PMID: 28104494). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag20077 Product name: Recombinant human PGRMC2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 55-145 aa of BC016692 Sequence: LLNVALVALVLLGAYRLWVRWGRRGLGAGAGAGEESPATSLPRMKKRDFSLEQLRQYDGSRNPRILLAVNGKVFDVTKGSKFYGPAGPYGI Predict reactive species Full Name: progesterone receptor membrane component 2 Calculated Molecular Weight: 223 aa, 24 kDa Observed Molecular Weight: 24 kDa, 28 kDa GenBank Accession Number: BC016692 Gene Symbol: PGRMC2 Gene ID (NCBI): 10424 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O15173 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.