Proteintech, 86874-1-RR, GPNMB Recombinant monoclonal antibody

Size: 20ul / 100ul The GPNMB (86874-1-RR) by Proteintech is a Recombinant antibody targeting GPNMB in WB, ELISA applications with reactivity to mouse, rat samples 86874-1-RR targets GPNMB in WB, ELISA applications and shows reactivity with mouse, rat samples. Tested Applications Positive WB detected in: RAW 264.7 cells, mouse skin tissue, rat skin tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro. Specification Tested Reactivity: mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg2814 Product name: Recombinant Mouse GPNMB protein (rFc Tag) Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 23-502 aa of NM_053110.4 Sequence: KRFRDVLGHEQYPDHMREHNQLRGWSSDENEWDEHLYPVWRRGDGRWKDSWEGGRVQAVLTSDSPALVGSNITFVVNLVFPRCQKEDANGNIVYEKNCRNDLGLTSDLHVYNWTAGADDGDWEDGTSRSQHLRFPDRRPFPRPHGWKKWSFVYVFHTLGQYFQKLGRCSARVSINTVNLTAGPQVMEVTVFRRYGRAYIPISKVKDVYVITDQIPVFVTMSQKNDRNLSDEIFLRDLPIVFDVLIHDPSHFLNDSAISYKWNFGDNTGLFVSNNHTLNHTYVLNGTFNLNLTVQTAVPGPCPPPSPSTPPPPSTPPSPPPSPLPTLSTPSPSLMPTGYKSMELSDISNENCRINRYGYFRATITIVEGILEVSIMQIADVPMPTPQPANSLMDFTVTCKGATPMEACTIISDPTCQIAQNRVCSPVAVDGLCLLSVRRAFNGSGTYCVNFTLGDDASLALTSTLISIPGKDPDSPLRAVN Predict reactive species Full Name: glycoprotein (transmembrane) nmb Calculated Molecular Weight: 64 kDa Observed Molecular Weight: 90-100 kDa, 120kDa GenBank Accession Number: NM_053110.4 Gene Symbol: Gpnmb Gene ID (NCBI): 93695 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q99P91 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.