Proteintech, 86883-1-RR, CCDC28A Recombinant monoclonal antibody

Size: 20ul / 100ul The CCDC28A (86883-1-RR) by Proteintech is a Recombinant antibody targeting CCDC28A in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 86883-1-RR targets CCDC28A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: MCF-7 cells, Jurkat cells, A375 cells, mouse thymus tissue, mouse testis tissue, rat testis tissue Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Coiled-coil domain-containing protein 28A (CCDC28A) is a translocation partner of nucleoporin 98 (NUP98) in acute leukemias, resulting in the creation of fusion genes and the expression of chimeric proteins. These fusion proteins, such as NUP98-CCDC28A, have been shown to promote myeloproliferative neoplasms (PMID: 22058212). Additionally, CCDC28A is essential for head-tail coupling stability, thereby ensuring sperm motility (PMID: 39500989). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag6529 Product name: Recombinant human CCDC28A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-162 aa of BC000758 Sequence: MPKKNAIPVSKSTGFSNPASQSTSQRPKLKRVMKEKTKPQGGEGKGAQSTPIQHSFLTDVSDVQEMERGLLSLLNDFHSGKLQAFGNECSIEQMEHVRGMQEKLARLNLELYGELEELPEDKRKTASDSNLDRLLSDLEELNSSIQKLHLADAQDVPNTSAS Predict reactive species Full Name: coiled-coil domain containing 28A Calculated Molecular Weight: 30 kDa Observed Molecular Weight: 25 kDa GenBank Accession Number: BC000758 Gene Symbol: CCDC28A Gene ID (NCBI): 25901 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q8IWP9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.