Proteintech, 87012-1-RR, JAB1 Recombinant monoclonal antibody

Size: 20ul / 100ul The JAB1 (87012-1-RR) by Proteintech is a Recombinant antibody targeting JAB1 in WB, ELISA applications with reactivity to human, mouse samples 87012-1-RR targets JAB1 in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, U-87 MG cells, MCF-7 cells, HCT 116 cells, HEK-293 cells, NIH/3T3 cells, PC-12 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Background Information Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Promotes the proteasomal degradation of BRSK2. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag25950 Product name: Recombinant human JAB1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-334 aa of BC001187 Sequence: MAASGSGMAQKTWELANNMQEAQSIDEIYKYDKKQQQEILAAKPWTKDHHYFKYCKISALALLKMVMHARSGGNLEVMGLMLGKVDGETMIIMDSFALPVEGTETRVNAQAAAYEYMAAYIENAKQVGRLENAIGWYHSHPGYGCWLSGIDVSTQMLNQQFQEPFVAVVIDPTRTISAGKVNLGAFRTYPKGYKPPDEGPSEYQTIPLNKIEDFGVHCKQYYALEVSYFKSSLDRKLLELLWNKYWVNTLSSSSLLTNADYTTGQVFDLSEKLEQSEAQLGRGSFMLGLETHDRKSEDKLAKATRDSCKTTIEAIHGLMSQVIKDKLFNQINIS Predict reactive species Full Name: COP9 constitutive photomorphogenic homolog subunit 5 (Arabidopsis) Calculated Molecular Weight: 37 kDa Observed Molecular Weight: 35-38 kDa GenBank Accession Number: BC001187 Gene Symbol: JAB1 Gene ID (NCBI): 10987 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q92905 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.