Proteintech, 87055-4-RR, Cleaved Caspase 3/P12 Recombinant monoclonal antibody

Size: 20ul / 100ul The Cleaved Caspase 3/P12 (87055-4-RR) by Proteintech is a Recombinant antibody targeting Cleaved Caspase 3/P12 in WB, IF/ICC, ELISA applications with reactivity to human samples 87055-4-RR targets Cleaved Caspase 3/P12 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Staurosporine treated Jurkat cells Positive IF/ICC detected in: Staurosporine treated Jurkat cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information Cleaved Caspase-3 p12 is one of the two subunits of active Caspase-3 and plays a key catalytic role in the execution of apoptosis. Precursor cleavage of Caspase-3 ultimately forms an active heterotetramer composed of two p17 subunits and two p12 subunits. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: caspase 3, apoptosis-related cysteine peptidase Calculated Molecular Weight: 277 aa, 32 kDa Observed Molecular Weight: 12 kDa GenBank Accession Number: BC016926 Gene Symbol: Caspase 3 Gene ID (NCBI): 836 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P42574 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.