Proteintech, 87084-2-RR, Phospho-EGFR (Tyr1110) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-EGFR (Tyr1110) (87084-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-EGFR (Tyr1110) in WB, ELISA applications with reactivity to human samples 87084-2-RR targets Phospho-EGFR (Tyr1110) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: EGF treated A431 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information EGFR is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Phospho-EGFR (Tyr1110) is the phosphorylated, active form of the epidermal growth factor receptor (EGFR) at tyrosine 1110 within the C-terminal tail. This modification is catalyzed by EGFR itself or SRC-family kinases upon ligand (EGF, TGF-α) binding or oxidative stress. Phosphorylation at Tyr1110 creates a docking site for SH2-domain-containing proteins, propagating downstream signals through the RAS-RAF-MEK-ERK and PI3K-AKT pathways. Some scientists call EGFR Tyr1110 as EGFR Tyr1086, the site where the signaling peptide is removed. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) Calculated Molecular Weight: 1210 aa, 134 kDa Observed Molecular Weight: 175 kDa GenBank Accession Number: BC094761 Gene Symbol: EGFR Gene ID (NCBI): 1956 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P00533 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.