Proteintech, 87114-1-RR, CRHR1 Recombinant monoclonal antibody

Size: 20ul / 100ul The CRHR1 (87114-1-RR) by Proteintech is a Recombinant antibody targeting CRHR1 in WB, ELISA applications with reactivity to human, mouse, rat samples 87114-1-RR targets CRHR1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, SH-SY5Y cells, Y79 cells, HT-29 cells, rat brain tissue, mouse brain tissue, mouse liver tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Corticotropin-releasing hormone (CRH, also known as Corticotropin-releasing factor, CRF) is a 41 amino acid peptide hormone and a key regulator of the stress response. CRH and the related urocortin peptides (UCN, UCN2 and UCN3) mediate their actions through two mammalian receptor subtypes, CRHR1 and CRHR2 (PMID: 23863939). CRHR1 (also known as CRFR1) exhibits high affinity towards CRH and UCN but low affinity towards UCN2 and no affinity towards UCN3 (PMID: 22684850). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Widely distributed throughout the brain, CRHR1 critically controls behavioral adaptation to stress and is causally linked to emotional disorders (PMID: 21885734). Alternative splicing results in multiple transcript variants encoding distinct isoforms of CRHR1. This antibody raised against a synthesized peptide corresponding to amino acid residues 148-165 of isoform CRF-R1 can only recognize isoform CRF-R1. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: corticotropin releasing hormone receptor 1 Calculated Molecular Weight: 48 kDa Observed Molecular Weight: 68 kDa GenBank Accession Number: NM_004382 Gene Symbol: CRHR1 Gene ID (NCBI): 1394 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P34998 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.