Proteintech, 87125-3-RR, NPTN Recombinant monoclonal antibody

Size: 20ul / 100ul The NPTN (87125-3-RR) by Proteintech is a Recombinant antibody targeting NPTN in WB, ELISA applications with reactivity to human, mouse, rat samples 87125-3-RR targets NPTN in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue, SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information Neuroplastin (Nptn) is a member of the Ig superfamily and is expressed in two isoforms, Np55 and Np65. Np65 contains three Ig domains (Ig1, Ig2, Ig3). Np55 contains only two (Ig2, Ig3). Np65 regulates synaptic transmission but the function of Np55 is unknown. Expression of the 65 kD neuroplastin (Np65) isoform derived by alternative splicing from the Nptn gene is restricted to brain neurons, whereas the 55 kD isoform (Np55) is present in most cell types but not in glia. (PMID: 33844052, PMID: 33299977, PMID: 27503893,PMID: 27581460) Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg5652 Product name: Recombinant human NPTN protein Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 29-339 aa of NM_012428.3 Sequence: QNAGFVKSPMSETKLTGDAFELYCDVVGSPTPEIQWWYAEVNRAESFRQLWDGARKRRVTVNTAYGSNGVSVLRITRLTLEDSGTYECRASNDPKRNDLRQNPSITWIRAQATISVLQKPRIVTSEEVIIRDSPVLPVTLQCNLTSSSHTLTYSYWTKNGVELSATRKNASNMEYRINKPRAEDSGEYHCVYHFVSAPKANATIEVKAAPDITGHKRSENKNEGQDATMYCKSVGYPHPDWIWRKKENGMPMDIVNTSGRFFIINKENYTELNIVNLQITEDPGEYECNATNAIGSASVVTVLRVRSHLAP Predict reactive species Full Name: neuroplastin Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: NM_012428.3 Gene Symbol: NPTN Gene ID (NCBI): 27020 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9Y639-2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.