Proteintech, 87164-1-RR, Phospho-EGFR (Tyr1172) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-EGFR (Tyr1172) (87164-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-EGFR (Tyr1172) in WB, IF/ICC, ELISA applications with reactivity to human samples 87164-1-RR targets Phospho-EGFR (Tyr1172) in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: EGF treated A431 cells, Serum-free treated and EGF treated A431 cells Positive IF/ICC detected in: EGF treated A431 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information ERBB1, also named as EGFR, is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Phospho-EGFR (Tyr1172) is a phosphorylated form of the epidermal growth factor receptor (EGFR), which is a transmembrane glycoprotein receptor that belongs to the ErbB family of receptor tyrosine kinases (RTKs). This phosphorylation event is significant as it activates downstream signaling cascades that regulate various cellular processes, including cell growth, proliferation, and survival. Some scientists call EGFR Tyr1172 as EGFR Tyr1148, the site where the signaling peptide is removed. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) Calculated Molecular Weight: 1210 aa, 134 kDa Observed Molecular Weight: 175 kDa GenBank Accession Number: BC094761 Gene Symbol: EGFR Gene ID (NCBI): 1956 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P00533 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.