Proteintech, 87174-1-RR, EBP50/NHERF-1 Recombinant monoclonal antibody

Size: 20ul / 100ul The EBP50/NHERF-1 (87174-1-RR) by Proteintech is a Recombinant antibody targeting EBP50/NHERF-1 in WB, ELISA applications with reactivity to human, rat samples 87174-1-RR targets EBP50/NHERF-1 in WB, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: HCT 116 cells, rat kidney tissue, HeLa cells, HepG2 cells, MCF-7 cells, MDA-MB-231 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Na+/H+ exchanger regulatory factor 1 (NHERF-1, also known as EBP50 ezrin-binding protein of 50 kDa, SLCA9A3R1) is a phosphoprotein that assembles multiprotein complexes via two PDZ domains and a C-terminal ezrin-binding domain. NHERF-1 was initially recognized as a scaffolding protein that recruits membrane transporters/receptors and cytoplasmic signaling proteins into functional complexes localized at or near the plasma membrane in epithelial cells (PMID: 19308292). Specification Tested Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag30878 Product name: Recombinant human SLC9A3R1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 260-358 aa of NM_004252 Sequence: TNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL Predict reactive species Full Name: solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 Calculated Molecular Weight: 39 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: NM_004252 Gene Symbol: SLC9A3R1/EBP50/NHERF-1 Gene ID (NCBI): 9368 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O14745 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.