Proteintech, 87205-1-RR, Phospho-HER2/ErbB2 (Tyr1196) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-HER2/ErbB2 (Tyr1196) (87205-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-HER2/ErbB2 (Tyr1196) in WB, ELISA applications with reactivity to human samples 87205-1-RR targets Phospho-HER2/ErbB2 (Tyr1196) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human Neuregulin-1 treated T-47D cells, human Neuregulin-1 treated MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. Phospho-HER2/ErbB2 (Tyr1196) is a phosphorylated form of the HER2 protein, which is a receptor tyrosine kinase that plays a critical role in cell growth, proliferation, and survival. The phosphorylation of HER2 at Tyr1196 is associated with the activation of downstream signaling pathways. In breast cancer, HER2 is overexpressed and contributes to aggressive tumor growth and poor prognosis. Overexpression of Her2/ErbB2/Neu in cancer is often correlated with recurrent distant metastasis, although the mechanism still remains largely elusive. Tyr1139/Tyr1196 are autonomously phosphorylated, when Her2 is overexpressed. (PMID: 21966491) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) Calculated Molecular Weight: 1255 aa, 138 kDa Observed Molecular Weight: 185 kDa GenBank Accession Number: NM_004448 Gene Symbol: HER2/ErbB2 Gene ID (NCBI): 2064 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P04626 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.