Proteintech, 87254-1-RR, RPA2 Recombinant monoclonal antibody

Size: 20ul / 100ul The RPA2 (87254-1-RR) by Proteintech is a Recombinant antibody targeting RPA2 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 87254-1-RR targets RPA2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: rat testis tissue, HeLa cells, HEK-293 cells, Jurkat cells, MOLT-4 cells, THP-1 cells, mouse testis tissue Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Tr residues that are phosphorylated in sequential order in response to genotoxic stress. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag0690 Product name: Recombinant human RPA2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 80-270 aa of BC001630 Sequence: IRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE Predict reactive species Full Name: replication protein A2, 32kDa Calculated Molecular Weight: 29 kDa Observed Molecular Weight: 32 kDa GenBank Accession Number: BC001630 Gene Symbol: RPA2 Gene ID (NCBI): 6118 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P15927 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.