Proteintech, 87302-4-RR, ATP6V1B2 Recombinant monoclonal antibody

Size: 20ul / 100ul The ATP6V1B2 (87302-4-RR) by Proteintech is a Recombinant antibody targeting ATP6V1B2 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 87302-4-RR targets ATP6V1B2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, HeLa cells, A549 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information ATP6V1B2(V-type proton ATPase subunit B, brain isoform) is also named as ATP6B2, VPP3,HO57. It belongs to the ATPase alpha/beta chains family.V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.The quasi-ubiquitous ATP6V1B2 is one of 2 isoforms of ATP6V1 and is expressed in most cell types, where it plays a major role in organelle acidification (PMID:18667600). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag7174 Product name: Recombinant human ATP6V1B2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 162-511 aa of BC003100 Sequence: CRIYPEEMIQTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSNQLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFERNFIAQGPYENRTVFETLDIGWQLLRIFPKEMLKRIPQSTLSEFYPRDSAKH Predict reactive species Full Name: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 Calculated Molecular Weight: 57 kDa Observed Molecular Weight: 56-58 kDa GenBank Accession Number: BC003100 Gene Symbol: ATP6V1B2 Gene ID (NCBI): 526 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P21281 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.