Proteintech, 87312-1-RR, Phospho-P53 (Ser33) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-P53 (Ser33) (87312-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-P53 (Ser33) in WB, ELISA applications with reactivity to human samples 87312-1-RR targets Phospho-P53 (Ser33) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: etoposide treated T-47D cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. Phosphorylation of p53 at specific amino acid residues plays a crucial role in mediating its various functions, including its role as a transcription factor that regulates the expression of genes involved in the cellular response to stress and DNA damage. Upon severe DNA damage, p53 is phosphorylated at specific amino acid residues, becomes stabilized, and activated to induce apoptosis-related genes. To date, 10 kinases have been identified to phosphorylate p53 for apoptosis at specific serine/threonine residues in the N- and C-terminus domains. (PMID: 30191640) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: tumor protein p53 Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 53 kDa GenBank Accession Number: NM_000546 Gene Symbol: P53 Gene ID (NCBI): 7157 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P04637 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.