Proteintech, 87385-1-RR, NHE1 Recombinant monoclonal antibody

Size: 20ul / 100ul The NHE1 (87385-1-RR) by Proteintech is a Recombinant antibody targeting NHE1 in WB, ELISA applications with reactivity to human, mouse, rat samples 87385-1-RR targets NHE1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: NCI-H1299 cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information NHE1, also named as SLC9A1, encodes an Na+/H+ antiporter that is a membrane-bound enzyme involved in regulating pH of vertebrate cells. NHE1 regulates a number of cell behaviors, including adhesion, shape determination, migration, and proliferation (PMID:11807182). It is inhibited by the non-specific diuretic drug amiloride and activated by growth factors, mitogens, neurotransmitters, tumor promoters, and others (PMID: 2846238). NHE1 is expressed in heart where it contributes to cardiomyocyte pH homeostasis (PMID: 12832126, PMID: 23709596). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: solute carrier family 9 (sodium/hydrogen exchanger), member 1 Calculated Molecular Weight: 815 aa, 91 kDa Observed Molecular Weight: 90-100 kDa GenBank Accession Number: BC012121 Gene Symbol: NHE1 Gene ID (NCBI): 6548 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P19634 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.