Proteintech, 87392-1-RR, PABPN1 Recombinant monoclonal antibody

Size: 20ul / 100ul The PABPN1 (87392-1-RR) by Proteintech is a Recombinant antibody targeting PABPN1 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 87392-1-RR targets PABPN1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, Jurkat cells, SH-SY5Y cells, MCF-7 cells, 4T1 cells, NIH/3T3 cells, PC-12 cells Positive IF/ICC detected in: NIH/3T3 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information Poly(A) Binding Protein Nuclear 1(PABPN1) is one of the isoforms of poly(A) binding protein (PABP) that locating to the nucleus in eukaryotic cells. And PABPN1 plays important role in mRNA stability by regulating of poly-A tail length, mRNA decay and proximal polyadenylation site (PAS) utilization at the 3'-UTR (PMID:23300856). Another report showed that PABPN1 shuttles between the nucleus and the cytoplasm to export poly(A) RNA from the nucleus (PMID:23601051). It is reported that PABPN1 is ubiquitously expressed in almost all tissues, however, mutations in PABPN1 causes Oculopharyngeal Muscular Dystrophy (OPMD) which affected in skeletal muscles specifically. Because of a mild reduction in PABPN1 levels is sufficient to induce muscle wasting (PMID:27152426). Acetylation, Methylation, Phosphoprotein are common post-translational modifications of PABPN1 protein. And 50-55 kDa bands have been reported (PMID: 29939290; 28361972). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag27814 Product name: Recombinant human PABPN1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 94-299 aa of BC010939 Sequence: GSQEEEEEPGLVEGDPGDGAIEDPELEAIKARVREMEEEAEKLKELQNEVEKQMNMSPPPGNAGPVIMSIEEKMEADARSIYVGNVDYGATAEELEAHFHGCGSVNRVTILCDKFSGHPKGFAYIEFSDKESVRTSLALDESLFRGRQIKVIPKRTNRPGISTTDRGFPRARYRARTTNYNSSRSRFYSGFNSRPRGRVYRGRARA Predict reactive species Full Name: poly(A) binding protein, nuclear 1 Calculated Molecular Weight: 33 kDa Observed Molecular Weight: 50-56 kDa GenBank Accession Number: BC010939 Gene Symbol: PABPN1 Gene ID (NCBI): 8106 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q86U42 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.