Proteintech, 87431-1-RR, HMBS Recombinant monoclonal antibody

Size: 20ul / 100ul The HMBS (87431-1-RR) by Proteintech is a Recombinant antibody targeting HMBS in WB, ELISA applications with reactivity to human samples 87431-1-RR targets HMBS in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, Caco-2 cells, L02 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information Hydroxymethylbilane synthase (HMBS), also known as porphobilinogen deaminase (PBGD) or previously as uroporphyrinogen I synthase, is a key enzyme in the heme biosynthesis pathway. HMBS is a cytoplasmic enzyme that catalyzes a critical step in the heme biosynthesis pathway: the polymerization of four porphobilinogen molecules to form linear hydroxymethylbilane. It is expressed in all tissues, with the highest levels observed in the liver and erythroid precursors to meet high heme demand. Two main isoforms exist: a ubiquitous "housekeeping" isoform and an erythroid-specific isoform. The observed molecular weight of human HMBS protein is approximately 40-42 kDa (as determined by SDS-PAGE). The erythroid-specific isoform has a slightly lower molecular weight due to a different translation initiation site. (PMID: 19292878, PMID: 12555854) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag6509 Product name: Recombinant human HMBS protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-361 aa of BC000520 Sequence: MSGNGNAAATAEENSPKMRVIRVGTRKSQLARIQTDSVVATLKASYPGLQFEIIAMSTTGDKILDTALSKIGEKSLFTKELEHALEKNEVDLVVHSLKDLPTVLPPGFTIGAICKRENPHDAVVFHPKFVGKTLETLPEKSVVGTSSLRRAAQLQRKFPHLEFRSIRGNLNTRLRKLDEQQEFSAIILATAGLQRMGWHNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTAMKDGQLYLTGGVWSLDGSDSIQETMQATIHVPAQHEDGPEDDPQLVGITARNIPRGPQLAAQNLGISLANLLLSKGAKNILDVARQLNDAH Predict reactive species Full Name: hydroxymethylbilane synthase Calculated Molecular Weight: 39 kDa Observed Molecular Weight: 42 kDa GenBank Accession Number: BC000520 Gene Symbol: HMBS Gene ID (NCBI): 3145 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P08397 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.