Proteintech, 87465-1-RR, Integrin beta 5 Recombinant monoclonal antibody

Size: 20ul / 100ul The Integrin beta 5 (87465-1-RR) by Proteintech is a Recombinant antibody targeting Integrin beta 5 in WB, IF/ICC, ELISA applications with reactivity to mouse, rat samples 87465-1-RR targets Integrin beta 5 in WB, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples. Tested Applications Positive WB detected in: Neuro-2a cells, C6 cells, mouse lung tissue Positive IHC detected in: mouse colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: RAW 264.7 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Integrin beta-5 (ITGB5) is expected to be located in the plasma membrane and mitochondria, which is i ubiquitinated in placenta and gall bladder. ITGB5 is a member of integrins and was proved to be associated with pathological processes in several tumors (PMID: 34966851). It is reported that TGB5 is highly expressed in hepatocellular carcinoma (HCC), and miR-185 regulates the expression of β- catenin through the ITGB5-dependent manner and affects the proliferation and migration of HCC cells (PMID: 35223869). Specification Tested Reactivity: mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg10456 Product name: Recombinant mouse ITB5 protein Source: mammalian cells -derived, pHZ-KIsec-C-6*HIS Tag: C-Twin-Strep-Tag Domain: 10011459 of Sequence: \ Predict reactive species Full Name: integrin beta 5 Observed Molecular Weight: 100 kDa Gene Symbol: Itgb5 Gene ID (NCBI): 16419 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.