Proteintech, 87525-1-RR, Phospho-PFKFB2 (Ser483) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-PFKFB2 (Ser483) (87525-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-PFKFB2 (Ser483) in WB, ELISA applications with reactivity to human samples 87525-1-RR targets Phospho-PFKFB2 (Ser483) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: IGF-1 treated MCF-7 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information Phospho-PFKFB2 (Ser483) is the active-site-switching modification of the heart-type bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB2). Phosphorylation at serine 483, located in the C-terminal regulatory segment, is catalyzed mainly by Akt downstream of PI3K and is essential for maximal 2-kinase activity: it increases the synthesis of fructose-2,6-bisphosphate (F2,6BP), the most potent allosteric activator of 6-phosphofructo-1-kinase (PFK-1), thereby accelerating glycolytic flux and ATP production. (PMID: 34282152) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 Calculated Molecular Weight: 58 kDa Observed Molecular Weight: 55 kDa GenBank Accession Number: NM_006212 Gene Symbol: PFKFB2 Gene ID (NCBI): 5208 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O60825 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.