Proteintech, 87549-1-RR, FBX4 Recombinant monoclonal antibody

Size: 20ul / 100ul The FBX4 (87549-1-RR) by Proteintech is a Recombinant antibody targeting FBX4 in WB, IF/ICC, ELISA applications with reactivity to human, mouse samples 87549-1-RR targets FBX4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HL-60 cells, HeLa cells, A431 cells, NIH/3T3 cells, mouse lung tissue, mouse thymus tissue, mouse spleen tissue Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information FBX4 (F-Box Protein 4), also known as FBXO4, is a member of the F-box protein family. FBX4 is involved in the ubiquitination of various target proteins, leading to their degradation. It has been shown to regulate the cell cycle by targeting proteins such as cyclin D1 for ubiquitination and degradation. Mutations in FBX4 have been linked to various diseases, including spermatogenic failure and esophageal cancer. In cancer, FBX4 has been shown to act as a tumor suppressor. Loss of FBX4 function can lead to increased cell proliferation and tumor development. For example, FBX4 knockout mice have been shown to develop various tumors, including lymphoblastic lymphoma and mammary carcinoma. FBX4 has two isforms: 44 kDa and 35 kDa. The observd molecular weight is consistent with the result in PMID: 21242966. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag5013 Product name: Recombinant human FBX4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-387 aa of BC048098 Sequence: MAGSEPRSGTNSPPPPFSDWGRLEAAILSGWKTFWQSVSKERVARTTSREEVDEAASTLTRLPIDVQLYILSFLSPHDLCQLGSTNHYWNETVRDPILWRYFLLRDLPSWSSVDWKSLPDLEILKKPISEVTDGAFFDYMAVYRMCCPYTRRASKSSRPMYGAVTSFLHSLIIQNEPRFAMFGPGLEELNTSLVLSLMSSEELCPTAGLPQRQIDGIGSGVNFQLNNQHKFNILILYSTTRKERDRAREEHTSAVNKMFSRHNEGDDQQGSRYSVIPQIQKVCEVVDGFIYVANAEAHKRHEWQDEFSHIMAMTDPAFGSSGRPLLVLSCISQGDVKRMPCFYLAHELHLNLLNHPWLVQDTEAETLTGFLNGIEWILEEVESKRAR Predict reactive species Full Name: F-box protein 4 Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 48 kDa GenBank Accession Number: BC048098 Gene Symbol: FBXO4/FBX4 Gene ID (NCBI): 26272 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9UKT5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.