Proteintech, 98030-1-RR, Anti-Human E-cadherin Rabbit Recombinant Antibody

Size: 100ug The E-cadherin (98030-1-RR) by Proteintech is a Recombinant antibody targeting E-cadherin in FC applications with reactivity to human samples 98030-1-RR targets E-cadherin in FC applications and shows reactivity with human samples. Tested Applications Positive FC detected in: MCF-7 cells, HT-29 cells Recommended dilution Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension Background Information Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CD324, CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag14973 Product name: Recombinant human E-cadherin protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 373-622 aa of BC141838 Sequence: PIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPI Predict reactive species Full Name: cadherin 1, type 1, E-cadherin (epithelial) Calculated Molecular Weight: 882 aa, 97 kDa GenBank Accession Number: BC141838 Gene Symbol: E-cadherin Gene ID (NCBI): 999 RRID: AB_3672175 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P12830 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.