Product Description
Size: 100ug
The CD45 (98117-1-RR) by Proteintech is a Recombinant antibody targeting CD45 in IHC, IF/ICC, FC applications with reactivity to human samples
98117-1-RR targets CD45 in IHC, IF/ICC, FC applications and shows reactivity with human samples.
Tested Applications
Positive IHC detected in: human tonsil tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: Jurkat cells
Positive FC detected in: human peripheral blood leukocytes
Recommended dilution
Immunohistochemistry (IHC): IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension
Background Information
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).
Specification
Tested Reactivity: human
Host / Isotype: Rabbit / IgG
Class: Recombinant
Type: Antibody
Immunogen: CatNo: Eg2486 Product name: recombinant human CD45(220-577) protein Source: mammalian cells -derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 10007019 of Sequence: Predict reactive species
Full Name: protein tyrosine phosphatase, receptor type, C
Calculated Molecular Weight: 147 kDa
Gene Symbol: CD45
Gene ID (NCBI): 5788
ENSEMBL Gene ID: ENSG00000081237
RRID: AB_3672264
Conjugate: Unconjugated
Form: Liquid
Purification Method: Protein A purfication
UNIPROT ID: P08575
Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.
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Collaboration
Tony Tang
Email: Tony.Tang@iright.com
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