Proteintech, 98136-1-RR, Rabbit IgG Isotype Control Recombinant Antibody

Size: 100ug The IgG Isotype Control (98136-1-RR) by Proteintech is a Recombinant antibody targeting IgG Isotype Control in WB, IHC, IF/ICC, FC, FC (Intra), IP, ELISA, ChIP-qPCR applications with reactivity to n/a samples 98136-1-RR targets IgG Isotype Control in WB, IHC, IF/ICC, FC, FC (Intra), IP, CoIP, RIP, ELISA, ChIP-qPCR applications and shows reactivity with n/a samples. Tested Applications Positive IHC detected in: Note: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive ChIP-qPCR detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.2 ug per 10^6 cells in 100 μl suspension Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody. Specification Tested Reactivity: n/a Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Small molecule Predict reactive species Full Name: IgG Isotype Control RRID: AB_3672282 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.