Proteintech, 98436-3-RR, Anti-Mouse CD53 Rabbit Recombinant Antibody

Size: 100ug The CD53 (98436-3-RR) by Proteintech is a Recombinant antibody targeting CD53 in FC applications with reactivity to mouse samples 98436-3-RR targets CD53 in FC applications and shows reactivity with mouse samples. Tested Applications Positive FC detected in: mouse splenocytes Recommended dilution Flow Cytometry (FC): FC : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information CD53 is also named as MOX44 and Tetraspanin-25 (Tspan-25). Tetraspanin CD53 is a member of the tetraspanin superfamily expressed exclusively within the immune compartment (PMID: 32440787). T cells depend on the phosphatase CD45 to initiate T cell receptor signaling. CD53 is shown to stabilize CD45 on the membrane and is required for optimal phosphatase activity and subsequent Lck activation. Together, CD53 as a regulator of CD45 activity required for T cell immunity (PMID: 35767951). CD53 is a marker for positively selected CD4+ CD8+ thymocytes (PMID: 11867561). Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg2684 Product name: Recombinant Mouse CD53 protein (rFc Tag)(HPLC verified) Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 107-181 aa of NM_007651.3 Sequence: EQKLNTLVAEGLNDSIQHYHSDNSTMKAWDFIQTQLQCCGVNGSSDWTSGPPSSCPSGADVQGCYNKAKSWFHSN Predict reactive species Full Name: CD53 antigen Calculated Molecular Weight: 24 kDa GenBank Accession Number: NM_007651.3 Gene Symbol: Cd53 Gene ID (NCBI): 12508 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q61451 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.