Proteintech, 98685-3-RR, Anti-Human EPHB4 Rabbit Recombinant Antibody

Size: 100ug The EPHB4 (98685-3-RR) by Proteintech is a Recombinant antibody targeting EPHB4 in FC applications with reactivity to human samples 98685-3-RR targets EPHB4 in FC applications and shows reactivity with human samples. Tested Applications Positive FC detected in: MCF-7 cells Recommended dilution Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information EPHB4 (Ephrin type-B receptor 4), a member of the largest family of receptor tyrosine kinases (RTK), is overexpressed in several tumor types, including prostate, breast, and bladder tumors and down-regulation of EPHB4 in tumor cell lines of these origins results in reduced cell viability, migration, and invasion. In endothelial cells, where EphB4 is normally expressed, it regulates cell migration, proliferation, and invasion (PMID: 15930280). The discovery of EphB4 as an alternative Epo receptor has multiple clinical implications including opportunities for patient stratification and anti-EphB4 approaches to abrogate the stimulatory effects of Epo on tumor growth (PMID: 16982731, 16424904). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg6504 Product name: Recombinant Human EPHB4 protein (rFc Tag) Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 16-539 aa of NM_004444.4 Sequence: LEETLLNTKLETADLKWVTFPQVDGQWEELSGLDEEQHSVRTYEVCDVQRAPGQAHWLRTGWVPRRGAVHVYATLRFTMLECLSLPRAGRSCKETFTVFYYESDADTATALTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNVKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCAQLTVNLTRFPETVPRELVVPVAGSCVVDAVPAPGPSPSLYCREDGQWAEQPVTGCSCAPGFEAAEGNTKCRACAQGTFKPLSGEGSCQPCPANSHSNTIGSAVCQCRVGYFRARTDPRGAPCTTPPSAPRSVVSRLNGSSLHLEWSAPLESGGREDLTYALRCRECRPGGSCAPCGGDLTFDPGPRDLVEPWVVVRGLRPDFTYTFEVTALNGVSSLATGPVPFEPVNVTTDREVPPAVSDIRVTRSSPSSLSLAWAVPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESEGWREQLA Predict reactive species Full Name: EPH receptor B4 Calculated Molecular Weight: 108 kDa GenBank Accession Number: NM_004444.4 Gene Symbol: EPHB4 Gene ID (NCBI): 2050 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P54760 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.