Proteintech, CL594-84590, CoraLite®594-conjugated Phospho-CRKL (Tyr207) Recombinant monoclonal antibody

Size: 100ul The Phospho-CRKL (Tyr207) (CL594-84590) by Proteintech is a Recombinant antibody targeting Phospho-CRKL (Tyr207) in FC (Intra) applications with reactivity to human, mouse samples CL594-84590 targets Phospho-CRKL (Tyr207) in FC (Intra) applications and shows reactivity with human, mouse samples. Tested Applications Positive FC (Intra) detected in: λ phosphatase treated K-562 cells Recommended dilution Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Similar to other adaptor proteins, Crk functions primarily to assemble molecular complexes to distribute signals within the cell. The best studied regulatory mechanism of Crk proteins is the phosphorylation at a tyrosine located in a linker between the N- and C-terminal SH3 domains (Tyr221 in human CrkII, Tyr207 in human CrkL). (PMID: 24675776) Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: v-crk sarcoma virus CT10 oncogene homolog (avian)-like Calculated Molecular Weight: 34 kDa Observed Molecular Weight: 39 kDa GenBank Accession Number: BC043500 Gene Symbol: CRKL Gene ID (NCBI): 1399 Conjugate: CoraLite®594 Fluorescent Dye Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm Form: Liquid Purification Method: Protein A purification UNIPROT ID: P46109 Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage.