Proteintech, PE-FcA65613, FcZero-rAb™ PE Anti-Human CD45RO (UCHL1) Rabbit IgG Recombinant Antibody

Size: 100tests The CD45RO (PE-FcA65613) by Proteintech is a Recombinant antibody targeting CD45RO in FC applications with reactivity to human samples PE-FcA65613 targets CD45RO in FC applications and shows reactivity with human samples. Tested Applications Positive FC detected in: human PBMCs Recommended dilution Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension Background Information CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: IL-2 dependent T cell line, CA1 Predict reactive species Full Name: protein tyrosine phosphatase, receptor type, C GenBank Accession Number: BC014239 Gene Symbol: CD45 Gene ID (NCBI): 5788 ENSEMBL Gene ID: ENSG00000081237 Conjugate: PE Fluorescent Dye Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm Excitation Laser: Blue Laser (488 nm)|Green Laser (532 nm)|Yellow-Green Laser (561 nm) Form: Liquid Purification Method: Protein A purification UNIPROT ID: P08575 Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.