Qiagen, 19597, Investigator Lyse&Spin Basket Kit (50)

50 pouches containing 50 baskets and 100 collection tubes,

Features

- Perform lysis and filtration of forensic samples on solid substrates
- Can be used for a variety of sample types
- Compatible with downstream manual and automated DNA extraction

Product Details

The Investigator Lyse&Spin Basket Kit is used to perform lysis and filtration of forensic samples on solid substrates. The procedure can be used for swabs, pieces of fabric or leather, paper, cigarette butts, chewing gum, small tapes, or other sample types. The Investigator Lyse&Spin Basket Kits meet ISO 18385 requirements.

Principle

Lyse&Spin baskets are used for pretreatment of forensic samples in combination with manual and automated extraction kits, for example, EZ1&2 DNA Investigator, QIAsymphony DNA Investigator, or QIAamp DNA Investigator Kits. They permit the combination of sample lysis and separation of solid sample substrates in one simple procedure. No sample transfer is required to obtain a cleared lysate.

Procedure

The basket retains the lysis buffer during the lysis step of the forensic sample. Upon centrifugation on an ordinary bench top centrifugation system, holes in the bottom of the basket open up and let the sample lysate pass through the basket. The lysate, which contains nucleic acids, is efficiently recovered and collected in the provided 2 ml collection tube, while the solid particles remain in the basket. Used baskets with solid sample substrates can optionally be stored in the 2nd collection tube provided (for Investigator Lyse&Spin Basket Kit (50) only).