Qiagen, 290145, mericon MeatTracker Kit (96)

For 96 reactions: PCR Assay Meat, Control DNA, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-Free Water, 50x ROX Dye Solution

Features

- Highly sensitive and specific meat identification, based on simple-real-time PCR
- Dedicated meat species-specific ID kits available
- Streamlined protocol, PCR run time of 73 minutes on the QIAGEN Rotor-Gene Q
- Dedicated mericon DNA isolation kit available

Performance

Assay: Catalog number
Number of reactions: 96
mericon Cattle Kit: 292025
mericon Chicken Kit: 292035
mericon Pig Kit: 292015
mericon Sheep Kit: 292065

Species: Result
Cattle: +
Chicken: +
Goat: +
Goose: +
Horse: +
Moose: +
Ostrich: +
Pig Wild boar: + +
Rabbit: +
Sheep: +
Turkey: +
Water buffalo: +
Corn: –
Potato: –
Rice: –
Soy: –
Wheat: –

The mericon MeatTracker Kit is a ready-to-use system for detection of specific DNA fragments from animal species in food, animal feed and pharmaceutical products using real-time polymerase chain reaction (PCR). The mericon MeatTracker Kit is designed for use with one or more of the following mericon Animal Identification Assays (see the table " mericon Animal ID Assays").

Specificity The mericon MeatTracker Kit exhibits high specificity for animal species DNA. The kit was tested against an extensive inclusivity panel of various vertebrate species and an exclusivity panel of non-vertebrate species. No cross-reactivity was observed with species using 2500 copies of tested DNA (see table "Results from cross-reactivity experiments with the mericon MeatTracker Kit).

Principle

Animal species detection by polymerase chain reaction (PCR) is based on the amplification of a specific region of the relevant species genome. In real-time PCR, the amplified product is detected via target-specific fluorescent probes that bind to the amplified product. Accumulation of PCR product results in increased fluorescent signal from the bound probes. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating PCR product without having to re-open the reaction tubes after the PCR run.

The probes of mericon PCR Assays are sequence-specific oligonucleotides with a fluorophore and a quencher moiety attached. The fluorophore is at the 5' end of the probe, and the quencher moiety is located at the 3' end. If the target DNA sequence is present, the probe is cleaved by the 5'-->3' exonuclease activity of HotStarTaq Plus DNA Polymerase during the extension phase of PCR. This separates the fluorophore and the quencher moiety, resulting in a detectable fluorescence that is proportional to the amount of accumulated PCR product.

The PCR primer set for each assay is highly specific and targets a unique and conserved DNA region of the tested species genome that is verified bioinformatically and experimentally. Cross-reactivity has been bioinformatically investigated and thoroughly tested with a panel of selected targets for each mericon PCR Assay. Each assay can detect as few as 10 target copies of each target in a reaction.

Procedure

DNA is extracted from a sample of food or animal feed using a dedicated sample preparation kit. QIAGEN's DNeasy mericon Food Kit is suitable for a whole range of food matrices, including those with high inhibitor contents.

The isolated, pure DNA is assayed using a streamlined real-time PCR protocol that returns results in 73 minutes on QIAGEN's Rotor-Gene Q. The protocol has also been validated for use on other thermal block cyclers.

The results are displayed as easy-to-interpret PCR curves.