Qiagen, 330451, RT2 PreAMP cDNA Synthesis Kit (12)

RT2 Nano PreAMP cDNA Synthesis Kit

Features

- Preamplification from nanogram amounts of RNA
- Amplification of 89 gene-specific cDNA targets
- Minimal nonspecific reactions in downstream PCR arrays

Procedure

- First strand cDNA synthesis: The kit can be used to synthesize cDNA from up to 12 different RNA samples. The built-in external RNA control template, which is detected by the reverse transcription control (RTC) assays in the RT² Profiler PCR Arrays, allows the detection of reverse transcription inhibitors and ensures efficient first strand synthesis.
- Preamplification of cDNA for pathway-specific genes: Each first strand cDNA synthesis reaction from 1–100 ng of total RNA can be amplified using up to 4 different sets of PCR array-specific primer mixes, allowing gene expression analysis on up to 4 different RT² Profiler PCR Arrays. The Side Reaction Reducer included in the kit eliminates the residual primers from preamplification, enabling accurate detection on PCR arrays.