Qiagen, 382426, Investigator 24plex GO! Kit (200)

Primer Mix, Fast Reaction Mix 2.0 including Taq DNA polymerase, Control DNA, allelic ladder 24plex, DNA size standard 24plex (BTO)

Features

- CODIS and European standard markers in one reaction
- High sensitivity and robustness with casework samples
- Direct amplification technology for reference samples
- Integrated Quality Sensor to support decision making
- PCR speed of around 45–60 minutes for faster results

Product Details

Investigator 24plex Kits are for human identification, allowing multiplex amplification of the CODIS (Combined DNA Index System) core loci, the ESS (European Standard Set) markers, plus SE33, DYS391 and Amelogenin. The kits include an innovative Quality Sensor, enabling invaluable additional data to be generated for quality control and performance tests. Furthermore, the Investigator 24plex GO! Kit enables direct amplification from reference samples without prior DNA extraction.

Investigator 24plex Kits meet ISO 18385 requirements.

Want to try the Investigator 24plex GO! Kit for the first time? Request a quote for a trial kit .

Want to try the Investigator 24plex QS Kit for the first time? Request a quote for a trial kit .

Performance

Investigator 24plex Kits use fast-cycling technology to provide reliable results in approximately 45–60 minutes.

The Fast Reaction Mix 2.0 is based on proven QIAGEN multiplex PCR technology. It has been further optimized for ease of handling and increased speed and sensitivity with the Investigator 24plex QS Kit and for direct amplification without prior DNA extraction with the Investigator 24plex GO! Kit. Assay performance tested in the presence of increasing concentrations of inhibitors shows superior inhibitor resistance. This enables reliable, high-quality results from casework samples, but also from direct amplification of typical reference samples such as blood or epithelial cells on FTA paper.

Investigator 24plex Kits were validated according to ENFSI and SWGDAM guidelines.

The recommended amount of DNA under standard conditions for the Investigator 24plex QS Kit is 0.5 ng. Internal validations demonstrated robust and balanced results with 0.2 to 2 ng DNA, and reliable results with <0.1 ng DNA.

Principle

- If amplification was successful
- If DNA was present in the sample
- If the sample was degraded
- If inhibitors in the sample prevented successful PCR

Investigator 24plex Kits use QIAGEN's fast-cycling PCR technology for rapid and reliable co-amplification of the CODIS and ESS markers, plus SE33, DYS391 and Amelogenin. Carefully designed primers reduce overlap of loci alleles, thereby minimizing the likelihood of data misinterpretation.

The innovative Quality Sensors provide additional information on sample quality and PCR success beyond the profile of the sample. With two Quality Sensors being amplified as a positive control, it is possible to determine:

This additional information will greatly enhance your data analysis and will help you to make crucial decisions with respect to further sample processing. The Quality Sensors will help you reduce unnecessary re-runs, saving you time and money.

The two gender markers, Amelogenin and DYS391, have been optimized to produce small fragment sizes, making the gender typing extremely robust. Even for populations with Amelogenin-null mutations, this means no more failures with the essential gender typing, even with degraded or inhibited samples.

Procedure

Dye: Marker
6-FAM: Amelogenin
BTG: TPOX
BTY: D10S1248
BTR2: D2S441
BTP: QS1

The 22 STR markers, the gender-specific Amelogenin and the Quality Sensor are amplified simultaneously in a single PCR and labeled with one of the dyes listed in the table "Investigator 24plex Kit dyes and corresponding markers".

The amplified loci then undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as Applied Biosystems GeneMapper ID-X . See the table "Technical specifications" for more information.

Applications

- Criminal database and forensic casework samples
- Paternity testing
- Population genetics studies
- Other human identification and forensics applications

Investigator 24plex Kits are for rapid and reliable generation of DNA profiles from blood, buccal swabs and forensic stains or direct and reliable generation of DNA profiles from blood on FTA or other paper, buccal cells on FTA or other paper or buccal swabs. They are highly suited for: